Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Name: Anti-Fatty Acid Synthase Antibody [ARC53122]
Brand: Antibodies.com
SKU: A308364

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This product is discontinued

Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) has been discontinued and is no longer available.

View all Anti-Fatty Acid Synthase Antibodies.

Specifications

Name

Anti-Fatty Acid Synthase Antibody [ARC53122]

Description

Rabbit monoclonal [ARC53122] antibody to Fatty Acid Synthase.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).

Sequence

QQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53122

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

273 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Enoyl-[acyl-carrier-protein] reductase, FAS, FASN, FAS_HUMAN, MGC14367, MGC15706, OA 519, Oleoyl-[acyl-carrier-protein] hydrolase, SDR27X1, Short chain dehydrogenase/reductase family 27X member 1, [Acyl-carrier-protein] S acetyltransferase, [Acyl-carrier-protein] S malonyltransferase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of 293F cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of Rat liver, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 4: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Confocal imaging of HeLa cells using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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