Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365)

Name: Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125]
Brand: Antibodies.com
SKU: A308365

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This product is discontinued

Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365) has been discontinued and is no longer available.

View all Anti-Fatty Acid Synthase Antibodies.

Specifications

Name

Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125]

Description

Rabbit Oligoclonal [ARC53122 + ARC53125] antibody to Fatty Acid Synthase.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:10,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) Rabbit PolymAb® (NP_004095.4).

Sequence

QQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC53122 + ARC53125

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

273 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Enoyl-[acyl-carrier-protein] reductase, FAS, FASN, FAS_HUMAN, MGC14367, MGC15706, OA 519, Oleoyl-[acyl-carrier-protein] hydrolase, SDR27X1, Short chain dehydrogenase/reductase family 27X member 1, [Acyl-carrier-protein] S acetyltransferase, [Acyl-carrier-protein] S malonyltransferase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365)

Western blot analysis of various lysates, using Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365)

Immunofluorescence analysis of HeLa using Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365) at a dilution of 1:500 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365)

Immunofluorescence analysis of HepG2 using Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365) at a dilution of 1:500 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365)

Immunofluorescence analysis of NIH/3T3 using Anti-Fatty Acid Synthase Antibody [ARC53122 + ARC53125] (A308365) at a dilution of 1:500 (40x lens). DAPI was used to stain the cell nuclei (blue).

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