This Fatty Acid Synthetase Activity Assay Kit is a quantitative assay kit for measuring Fatty Acid Synthetase activity in serum, plasma, animal and plant tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Fatty acid synthase consumes NADPH during fatty acid synthesis from acetyl-CoA and malonyl-CoA. Activity is determined from the decrease in absorbance at 340 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
Extraction Buffer, Assay Buffer, NADPH, Acetyl CoA, Malonyl CoA
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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