Anti-FGF14 Antibody (A82593) (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0.5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method.
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