Anti-FGFR1 Antibody [ARC53424] (A306276)

Name: Anti-FGFR1 Antibody [ARC53424]
Brand: Antibodies.com
SKU: A306276
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53424] antibody to FGFR1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-FGFR1 Antibody [ARC53424]

Description

Rabbit monoclonal [ARC53424] antibody to FGFR1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y653 & Y654 of human FGFR1 (NP_075598.2).

Sequence

IDYYKK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53424

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

122 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Basic fibroblast growth factor receptor 1, bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR-1, FGFR1/PLAG1 fusion, FGFR1_HUMAN, fibroblast growth factor receptor 1, FLG, FLT-2, FLT2, Fms-like gene, Fms-like tyrosine kinase 2, fms-related tyrosine kinase 2, HBGFR, heparin-binding growth factor receptor, HH2, HRTFDS, hydroxyaryl-protein kinase, KAL2, N-SAM, OGD, Proto-oncogene c-Fgr

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-FGFR1 Antibody [ARC53424] (A306276)

Western blot analysis of U-251MG, using Anti-FGFR1 Antibody [ARC53424] (A306276) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-FGFR1 Antibody [ARC53424] (A306276)

Western blot analysis of various lysates, using Anti-FGFR1 Antibody [ARC53424] (A306276) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-FGFR1 Antibody [ARC53424] (A306276)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-FGFR1 Antibody [ARC53424] (A306276) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-FGFR1 Antibody [ARC53424] (A306276)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-FGFR1 Antibody [ARC53424] (A306276) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.