Anti-Fibrinogen Antibody (A282784)

Anti-Fibrinogen Antibody

Sheep polyclonal antibody to Fibrinogen.

This antibody recognises human fibrinogen, a complex ~340 kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of α, β and γ chains linked by a series of 29 disulphide bonds. The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-terminal ends of the α chains during fibrin clot cross-linking. Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse. Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA.

ELISA, IHC-Fr, IF

ELISA: 1:5,000 - 1:50,000, IF: 1:200 - 1:400

Human, Mouse, Rat

Human fibrinogen purified from plasma.

Sheep

Polyclonal

IgG

Unconjugated

5 mg/ml

Liquid

Supplied in Glycine Buffered Saline with <0.1% Sodium Azide, <0.1% EACA, <0.01% Benzamidine, and <1mM EDTA.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Sheep anti Human fibrinogen antibody recognizes human fibrinogen, a complex ~340 kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of a, ß and ? chains linked by a series of 29 disulphide bonds (Henschen et al. 1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the a chains during fibrin clot cross-linking. Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orino et al. 1993). Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashi et al. 2013).

FGA, Fib2, FIBA_HUMAN, Fibrinogen A alpha polypeptide, Fibrinogen alpha chain, Fibrinogen B alpha polypeptide, Fibrinogen beta chain, Fibrinogen G alpha polypeptide, Fibrinogen gamma chain, fibrinogen, B beta polypeptide, fibrinogen, G gamma polypeptide, fibrinogen, gamma polypeptide, Fibrinogen--alpha -polypeptide chain, Fibrinogen--beta -polypeptide chain, Fibrinogen--gamma-polypeptide chain