Anti-Fibrinogen Antibody [AB10-3C12] (Biotin) (A279624)

Anti-Fibrinogen Antibody [AB10-3C12] (Biotin)

Mouse monoclonal [AB10-3C12] antibody to Fibrinogen (Biotin).

This antibody recognises fibrinogen, a 340 kDa glycoprotein secreted by the liver which circulates in plasma as a soluble homodimer. The identical monomer subunits are linked by 17 disulfide bond and each subunit is composed of 3 polypeptide chains referred to as alpha, beta and gamma. In response to bleeding, the coagulation cascade is activated which results in the conversion of fibrinogen to fibrin. The resulting fibrin spontaneously forms polymers creating an insoluble gel which combines with platelets to form a spongy mass. This mass is the blood clot which subsequently hardens to prevent further bleeding from the blood vessel. The impairment of this conversion from fibrinogen to fibrin is associated with a range of pathologies including coagulopathies, ischemic stroke and obstetrical complications. It is also implicated in various neurological diseases such as Alzheimer’s, multiple sclerosis and traumatic central nervous system injury. The biotinylated Mouse anti Human fibrinogen antibody, clone AB10-3C12 (MCA6191B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human fibrinogen antibody, clone AB05-1F11 (MCA6190GA) as the capture antibody.

ELISA: 1 µg/ml

Human

Fibrinogen purified from human serum.

Mouse

Monoclonal

AB10-3C12

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human fibrinogen antibody, clone AB10-3C12 recognizes fibrinogen, a 340 kDa glycoprotein secreted by the liver which circulates in plasma as a soluble homodimer. The identical monomer subunits are linked by 17 disulfide bond and each subunit is composed of 3 polypeptide chains referred to as alpha, beta and gamma (Tiscia & Margaglione 2018). In response to bleeding, the coagulation cascade is activated which results in the conversion of fibrinogen to fibrin. The resulting fibrin spontaneously forms polymers creating an insoluble gel which combines with platelets to form a spongy mass. This mass is the blood clot which subsequently hardens to prevent further bleeding from the blood vessel (Tiscia & Margaglione 2018). The impairment of this conversion from fibrinogen to fibrin is associated with a range of pathologies including coagulopathies, ischemic stroke and obstetrical complications. It is also implicated in various neurological diseases such as Alzheimer’s, multiple sclerosis and traumatic central nervous system injury (Petersen et al. 2018).The biotinylated Mouse anti Human fibrinogen antibody, clone AB10-3C12 (MCA6191B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human fibrinogen antibody, clone AB05-1F11 (MCA6190GA) as the capture antibody.

FGA, Fib2, FIBA_HUMAN, Fibrinogen A alpha polypeptide, Fibrinogen alpha chain, Fibrinogen B alpha polypeptide, Fibrinogen beta chain, Fibrinogen G alpha polypeptide, Fibrinogen gamma chain, fibrinogen, B beta polypeptide, fibrinogen, G gamma polypeptide, fibrinogen, gamma polypeptide, Fibrinogen--alpha -polypeptide chain, Fibrinogen--beta -polypeptide chain, Fibrinogen--gamma-polypeptide chain

This product is for research use only. It is not intended for diagnostic or therapeutic use.