Anti-Fibroblasts/Epithelial Cells Antibody [D7-FIB] (PE) (A280220)

Anti-Fibroblasts/Epithelial Cells Antibody [D7-FIB] (PE)

Mouse monoclonal [D7-FIB] antibody to Fibroblasts/Epithelial Cells (PE).

The epitope recognized by clone D7-FIB is sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB also binds to epithelium, myoepthelium, smooth muscle and some leucocytes. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

This antibody does not cross react with Rat or Mouse

Human foreskin fibroblasts.

Mouse

Monoclonal

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water. Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Recognises a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines.

This product is for research use only. It is not intended for diagnostic or therapeutic use.