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Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)

Validation Data - Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)
$745
Product Datasheet

Mouse monoclonal [FN12-8] antibody to Fibronectin for WB, ELISA, Inhibition Assay and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Fibronectin Antibody [FN12-8] - Azide free
Description
Mouse monoclonal [FN12-8] antibody to Fibronectin.
Specificity
Recognizes an epitope in the cell-binding domain. Can inhibit cell adhesion.
Applications
WB, ELISA, Inhibition Assay, IHC
Dilutions
WB: 1-10 µg/ml, IHC-P: 5-10 µg/ml, IHC-Fr: 5-10 µg/ml, ELISA: 1-10 µg/ml, Cell Adhesion Inhibition Assay: 0.1-1 µg/ml
Reactivity
Human, Bovine
Immunogen
Human plasma Fibronectin.
Host
Mouse
Clonality
Monoclonal
Clone ID
FN12-8
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 200µl of double distilled water (2mg/ml final concentration).
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 1% BSA.
Storage
Lyophilized: Store at 4°C. Reconstituted: Aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
For IHC use proteinase K treatment for antigen retrieval.WB under reducing or non-reducing conditions.
Synonyms
CIG, Cold insoluble globulin , Cold-insoluble globulin, DKFZp686F10164 , DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B , Fibronectin 1, FINC, FINC_HUMAN, FN, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor , MSF, Ugl-Y3
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Validation Data - Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)Enlarge Image
Figure 1: Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)
IHC detection of Fibronectin using A304547 at 1-10µg/ml on paraffin-embedded colon cancer tissue following Proteinase K treatment for antigen retrieval. Staining of Fibronectin in human well-differentiated colon cancer tissue (lower) and moderately differentiated colon cancer (upper).
Publishing research using Anti-Fibronectin Antibody [FN12-8] - Azide free (A304547)? Please let us know so that we can list the citation on this page.

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