Mouse monoclonal [LUC-Y] antibody to Firefly Luciferase.
Specificity
This antibody recognises the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
Applications
WB, Flow Cytometry, IHC-Fr, ELISA, IF
Dilutions
ELISA: 1:500 - 1:5,000, FC 1: 10µg/ml, WB: 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Insect
Immunogen
Recombinant luciferase expressed in E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
LUC-Y
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 55-60 kDa with a sample of 1ug of purified luciferase from Photinus pyralis on a Western Blot.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
