Anti-FMRP Antibody [ARC1026] (A307211)

Name: Anti-FMRP Antibody [ARC1026]
Brand: Antibodies.com
SKU: A307211
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1026] antibody to FMRP for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-FMRP Antibody [ARC1026]

Description

Rabbit monoclonal [ARC1026] antibody to FMRP.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FMRP (Q06787).

Sequence

MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1026

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 - 80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FMR 1, Fmr1, Fmr1 gene, FMR1_HUMAN, Fragile X mental retardation 1, Fragile X mental retardation 1 protein, Fragile X mental retardation protein, Fragile X mental retardation protein 1, fragile X mental retardation syndrome-related protein 1, fragile X mental retardation, autosomal homolog 1, FRAXA, fxr1, MGC87458, POF, POF1, Protein FMR-1, Protein FMR1, wu:fb16f11, wu:fd18c10, zgc:66226

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-FMRP Antibody [ARC1026] (A307211)

Western blot analysis of extracts of various cell lines, using Anti-FMRP Antibody [ARC1026] (A307211) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-FMRP Antibody [ARC1026] (A307211)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-FMRP Antibody [ARC1026] (A307211) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-FMRP Antibody [ARC1026] (A307211)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-FMRP Antibody [ARC1026] (A307211) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-FMRP Antibody [ARC1026] (A307211)

Immunoprecipitation analysis of 300µg extracts of SH-SY5Y cells using 3µg of Anti-FMRP Antibody [ARC1026] (A307211). This Western blot was performed on the immunoprecipitate using Anti-FMRP Antibody [ARC1026] (A307211) at a dilution of 1:1000.

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