Anti-FOX2 Antibody (A104328)

Immunofluorescent analysis of mouse cerebellum section stained with Anti-FOX2 Antibody (1:2,000 | red), and co-stained with Anti-NF-L Antibody (1:5,000 | green). The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies. Anti-FOX2 Antibody reveals protein expressed in the nuclei of certain granule cells and all Purkinje cells, while Anti-NF-L Antibody labels dendrites and axons.

Western blot analysis of nuclear enriched fractions from different tissue and cells using Anti-FOX2 Antibody in green: [1] protein standard , [2] rat brain, [3] mouse brain, [4] HEK293, and [5] NIH-3T3 cells. The bands between 50kDa and 75kDa correspond to the FOX2 protein.