Anti-FOXA2 Antibody [ARC0391] (A307499)

Name: Anti-FOXA2 Antibody [ARC0391]
Brand: Antibodies.com
SKU: A307499
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0391] antibody to FOXA2 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-FOXA2 Antibody [ARC0391]

Description

Rabbit monoclonal [ARC0391] antibody to FOXA2.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HNF3ß/FOXA2 (Q9Y261).

Sequence

MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0391

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Forkhead box A2, Forkhead box protein A2, FOX A2, FOXA2_HUMAN, Hepatic nuclear factor 3 beta, Hepatocyte nuclear factor 3, Hepatocyte nuclear factor 3 beta, Hepatocyte nuclear factor 3-beta, HNF 3B, HNF-3-beta, HNF-3B, HNF3B, MGC19807, TCF 3B, TCF-3B, TCF3B, Transcription factor 3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-FOXA2 Antibody [ARC0391] (A307499)

Western blot analysis of extracts of various cell lines, using Anti-FOXA2 Antibody [ARC0391] (A307499) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-FOXA2 Antibody [ARC0391] (A307499)

Immunofluorescence analysis of Hep G2 cells using Anti-FOXA2 Antibody [ARC0391] (A307499) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-FOXA2 Antibody [ARC0391] (A307499)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-FOXA2 Antibody [ARC0391] (A307499). This Western blot was performed on the immunoprecipitate using Anti-FOXA2 Antibody [ARC0391] (A307499) at a dilution of 1:1000.

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