Anti-FOXO1A (phospho Ser256) Antibody (A16490)

Name: Anti-FOXO1A (phospho Ser256) Antibody
Brand: Antibodies.com
SKU: A16490

Enlarge Image

This product is discontinued

Anti-FOXO1A (phospho Ser256) Antibody (A16490) has been discontinued and is no longer available.

View all Anti-FOXO1A Antibodies.

Specifications

Name

Anti-FOXO1A (phospho Ser256) Antibody

Description

Rabbit polyclonal antibody to FOXO1A (phospho Ser256).

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S256 of human FoxO1 (NP_002006.2).

Sequence

AASMD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FKH 1, FKH1, FKHR, Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma), Forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, FoxO transcription factor, foxo1, FOXO1_HUMAN, OTTHUMP00000018301

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-FOXO1A (phospho Ser256) Antibody (A16490)

Western blot analysis of extracts of 293T cells, using Anti-FOXO1A (phospho Ser256) Antibody (A16490). 293T were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 2: Western Blot - Anti-FOXO1A (phospho Ser256) Antibody (A16490)

Western blot analysis of extracts of HeLa cells, using Anti-FOXO1A (phospho Ser256) Antibody (A16490) at 1:1,000 dilution or FOXO1 antibody (A13862). Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 2s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-FOXO1A (phospho Ser256) Antibody (A16490)

Immunofluorescence analysis of NIH/3T3 using Anti-FOXO1A (phospho Ser256) Antibody (A16490) at a dilution of 1:50. DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-FOXO1A (phospho Ser256) Antibody (A16490)

Immunofluorescence analysis of PC-12 using Anti-FOXO1A (phospho Ser256) Antibody (A16490) at a dilution of 1:50. DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-FOXO1A (phospho Ser256) Antibody (A16490)? Please let us know so that we can list the citation on this page.