Anti-FTO Antibody [ARC0851] (A307474)

Name: Anti-FTO Antibody [ARC0851]
Brand: Antibodies.com
SKU: A307474

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This product is discontinued

Anti-FTO Antibody [ARC0851] (A307474) has been discontinued and is no longer available.

View all Anti-FTO Antibodies.

Specifications

Name

Anti-FTO Antibody [ARC0851]

Description

Rabbit monoclonal [ARC0851] antibody to FTO.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FTO (Q9C0B1).

Sequence

MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0851

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AlkB homolog 9, ALKBH9, Alpha-ketoglutarate-dependent dioxygenase FTO, AW743446, Fat mass and obesity-associated protein, FATSO, MOUSE, HOMOLOG OF, FTO_HUMAN, GDFD, KIAA1752, mKIAA1752, Protein fatso

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-FTO Antibody [ARC0851] (A307474)

Western blot analysis of extracts of various cell lines, using Anti-FTO Antibody [ARC0851] (A307474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-FTO Antibody [ARC0851] (A307474)

Immunohistochemistry analysis of paraffin-embedded human bladder cancer using Anti-FTO Antibody [ARC0851] (A307474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-FTO Antibody [ARC0851] (A307474)

Immunofluorescence analysis of U-2 OS cells using Anti-FTO Antibody [ARC0851] (A307474) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-FTO Antibody [ARC0851] (A307474)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-FTO Antibody [ARC0851] (A307474). This Western blot was performed on the immunoprecipitate using Anti-FTO Antibody [ARC0851] (A307474) at a dilution of 1:500.

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