Anti-FTO Antibody [ARC52114] (A308821)

Name: Anti-FTO Antibody [ARC52114]
Brand: Antibodies.com
SKU: A308821

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This product is discontinued

Anti-FTO Antibody [ARC52114] (A308821) has been discontinued and is no longer available.

View all Anti-FTO Antibodies.

Specifications

Name

Anti-FTO Antibody [ARC52114]

Description

Rabbit monoclonal [ARC52114] antibody to FTO.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 327-504 of human FTO (NP_001073901.1).

Sequence

STGTLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52114

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AlkB homolog 9, ALKBH9, Alpha-ketoglutarate-dependent dioxygenase FTO, AW743446, Fat mass and obesity-associated protein, FATSO, MOUSE, HOMOLOG OF, FTO_HUMAN, GDFD, KIAA1752, mKIAA1752, Protein fatso

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-FTO Antibody [ARC52114] (A308821)

Western blot analysis of various lysates, using Anti-FTO Antibody [ARC52114] (A308821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 2: Western Blot - Anti-FTO Antibody [ARC52114] (A308821)

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Figure 3: Western Blot - Anti-FTO Antibody [ARC52114] (A308821)

Western blot analysis of extracts from normal (control) and FTO knockout (KO) 293T(KO) cells, using Anti-FTO Antibody [ARC52114] (A308821) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 4: Immunohistochemistry - Anti-FTO Antibody [ARC52114] (A308821)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-FTO Antibody [ARC52114] (A308821) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.