Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761)

Name: Anti-GABA A Receptor alpha 1 Antibody [N95/35]
Brand: Antibodies.com
SKU: A304761
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [N95/35] antibody to GABA A Receptor alpha 1 for WB, IHC, ICC/IF and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GABA A Receptor alpha 1 Antibody [N95/35]

Description

Mouse monoclonal [N95/35] antibody to GABA A Receptor alpha 1.

Applications

WB, IHC, ICC/IF, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with GABA A Receptor alpha 2 or GABA A Receptor alpha 3.

Immunogen

Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1.

Host

Mouse

Clonality

Monoclonal

Clone ID

N95/35

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S95-35.

Synonyms

ECA4, EIEE19, EJM, EJM5, Gaba receptor alpha 1 polypeptide, GABA(A) receptor, GABA(A) receptor subunit alpha 1, GABA(A) receptor subunit alpha-1, GABA(A) receptor, alpha 1, GABRA 1, GABRA1, Gamma aminobutyric acid (GABA) A receptor alpha 1, Gamma aminobutyric acid A receptor alpha 1, Gamma aminobutyric acid receptor subunit alpha 1, Gamma aminobutyric acid type A receptor alpha1 subunit, Gamma-aminobutyric acid receptor subunit alpha-1, GBRA1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761)

Western blot analysis of human Cell line lysates showing detection of GABA A Receptor protein using Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunohistochemistry - Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Dermal Cells.

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Figure 3: Immunofluorescence - Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GABA A Receptor alpha 1 Antibody [N95/35] (A304761), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.

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