Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759)

Name: Anti-GABA B Receptor 1 Antibody [S93A-49]
Brand: Antibodies.com
SKU: A304759
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S93A-49] antibody to GABA B Receptor 1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GABA B Receptor 1 Antibody [S93A-49]

Description

Mouse monoclonal [S93A-49] antibody to GABA B Receptor 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with GABA B Receptor 2.

Immunogen

Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S93A-49

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~115 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

dJ271M21.1.1, dJ271M21.1.2, FLJ92613, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABAB R1, GABAB subunit 1c, GABABR1, GABBR1, GABBR1 3, GABR1_HUMAN, Gamma aminobutyric acid (GABA) B receptor 1, Gamma-aminobutyric acid type B receptor subunit 1, Gb1, GPRC3A, Seven transmembrane helix receptor

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759)

Western blot analysis of rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GABA B Receptor 1 Antibody [S93A-49] (A304759), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-B Receptor 1 Antibody. (D) Composite.

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