Anti-GABRA4 Antibody [S398A-34] (A304799)

Name: Anti-GABRA4 Antibody [S398A-34]
Brand: Antibodies.com
SKU: A304799
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S398A-34] antibody to GABRA4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GABRA4 Antibody [S398A-34]

Description

Mouse monoclonal [S398A-34] antibody to GABRA4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with other GABA A Receptor alpha subunits.

Immunogen

Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4.

Host

Mouse

Clonality

Monoclonal

Clone ID

S398A-34

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GABA A Receptor alpha 4, GABA(A) receptor subunit alpha 4, GABA(A) receptor subunit alpha-4, GABR A4, GABRA 4, Gamma aminobutyric acid (GABA) A receptor alpha 4, Gamma aminobutyric acid A receptor alpha 4, Gamma aminobutyric acid receptor alpha 4 subunit, Gamma aminobutyric acid receptor subunit alpha 4, Gamma-aminobutyric acid receptor subunit alpha-4, GBRA4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GABRA4 Antibody [S398A-34] (A304799)

Western blot analysis of mouse brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Anti-GABRA4 Antibody [S398A-34] (A304799) at 1:1,000 for 16 hours at 4°C. Lane 1: MW Ladder. Lane 2: mouse Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~60 kDa.

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Figure 2: Immunofluorescence - Anti-GABRA4 Antibody [S398A-34] (A304799)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GABRA4 Antibody [S398A-34] (A304799), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha4 Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-GABRA4 Antibody [S398A-34] (A304799)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GABRA4 Antibody [S398A-34] (A304799), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.

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