Anti-GABRD Antibody [N151/3] (A305006)

Name: Anti-GABRD Antibody [N151/3]
Brand: Antibodies.com
SKU: A305006
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [N151/3] antibody to GABRD for WB, ICC/IF and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GABRD Antibody [N151/3]

Description

Mouse monoclonal [N151/3] antibody to GABRD.

Applications

WB, ICC/IF, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta.

Host

Mouse

Clonality

Monoclonal

Clone ID

N151/3

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S151-3.

Synonyms

GABA A Receptor delta, GABA(A) receptor subunit delta, Gamma aminobutyric acid GABA A receptor delta, Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor, Gamma-aminobutyric acid receptor subunit delta, GBRD_HUMAN, MGC45284

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GABRD Antibody [N151/3] (A305006)

Western blot analysis of rat Cell line lysates showing detection of GABA A Receptor protein using Anti-GABRD Antibody [N151/3] (A305006) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-GABRD Antibody [N151/3] (A305006)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-GABRD Antibody [N151/3] (A305006), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Diffuse cytoplasm and dull nuclei.

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Figure 3: Immunofluorescence - Anti-GABRD Antibody [N151/3] (A305006)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GABRD Antibody [N151/3] (A305006), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.

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