Anti-Galectin 1 Antibody [ARC53156] (A306835)

Name: Anti-Galectin 1 Antibody [ARC53156]
Brand: Antibodies.com
SKU: A306835

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This product is discontinued

Anti-Galectin 1 Antibody [ARC53156] (A306835) has been discontinued and is no longer available.

View all Anti-Galectin 1 Antibodies.

Specifications

Name

Anti-Galectin 1 Antibody [ARC53156]

Description

Rabbit monoclonal [ARC53156] antibody to Galectin 1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human Galectin 1/LGALS1 (NP_002296.1).

Sequence

MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53156

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding lectin, Beta galactoside binding lectin L 14 I, beta galactoside binding protein, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, Gal 1, Gal-1, GAL1, Galaptin, Galbp, Galectin, Galectin-1, Galectin1, GBP, HBL, HLBP14, HPL, L 14.5, L-14.5, L14, Lactose binding lectin 1, Lactose-binding lectin 1, Lect14, Lectin galactoside binding soluble 1, Lectin galactoside-binding soluble 1, LEG1_HUMAN, LGALS 1, LGALS1, Lgals1 lectin galactose binding soluble 1, MAPK activating protein MP12, Putative MAPK activating protein MP12, Putative MAPK-activating protein PM12, S Lac lectin 1, S-Lac lectin 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Galectin 1 Antibody [ARC53156] (A306835)

Western blot analysis of extracts of various cell lines, using Anti-Galectin 1 Antibody [ARC53156] (A306835) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Galectin 1 Antibody [ARC53156] (A306835)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Galectin 1 Antibody [ARC53156] (A306835) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Galectin 1 Antibody [ARC53156] (A306835)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Galectin 1 Antibody [ARC53156] (A306835) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Galectin 1 Antibody [ARC53156] (A306835)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Galectin 1 Antibody [ARC53156] (A306835) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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