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Recombinant Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121244)

Western Blot - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121244) - Antibodies.com Immunocytochemistry - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121266) - Antibodies.com
Recombinant Antibody
$585
Product Datasheet

Recombinant rabbit monoclonal [RM224] antibody to gamma H2A.X (phospho Ser139) for WB, ELISA, Multiplex Protein Detection and ICC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-gamma H2A.X (phospho Ser139) Antibody [RM224]
Description
Recombinant rabbit monoclonal [RM224] antibody to gamma H2A.X (phospho Ser139).
Specificity
This antibody recognizes Histone H2A.X only when phosphorylated at Serine 139.
Applications
WB, ELISA, Multiplex Protein Detection, ICC
Dilutions
WB: 0.5-2 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.1-1 µg/ml, ICC: 0.5-2 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other phosphorylated Histones.
Immunogen
Synthetic peptide corresponding to gamma Histone H2A.X (phospho Ser139).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM224
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121244) - Antibodies.comEnlarge Image

Western Blot - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121244)

Western blot of acid extracts of HeLa cells treated or non-treated with UV, using Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] at 0.5µg/ml, showed a band of Histone H2A.X phosphorylated at Serine 139 in HeLa cells.
Immunocytochemistry - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121266) - Antibodies.comEnlarge Image

Immunocytochemistry - Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (A121244)

Immunocytochemistry of HeLa cells using Anti-gamma H2A.X (phospho Ser139) Antibody [RM224] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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