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Recombinant Anti-gamma Heavy Chain Antibody [RM116] (A121312)

Immunohistochemistry - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.com ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.com ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121291) - Antibodies.com ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.com ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.com ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.com
Recombinant Antibody
$480

Recombinant rabbit monoclonal [RM116] antibody to gamma Heavy Chain for ICC, IHC, Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-gamma Heavy Chain Antibody [RM116]
Description
Recombinant rabbit monoclonal [RM116] antibody to gamma Heavy Chain.
Specificity
This antibody recognizes the Fc region of all gamma heavy chains of human immunoglobulins, including: ?1, ?2, ?3, and ?4.
Applications
ICC, IHC, Flow Cytometry, ELISA
Dilutions
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Reactivity
Human
Cross Reactivity
There is no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG.
Immunogen
Human IgG.
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM116
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-gamma Heavy Chain Antibody [RM116] (A121312)
Immunohistochemical staining of human tonsil using Anti-gamma Heavy Chain Antibody [RM116].
ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.comEnlarge Image
Figure 2: Anti-gamma Heavy Chain Antibody [RM116] (A121312)
ELISA of human immunoglobulins shows Anti-gamma Heavy Chain Antibody [RM116] reacted to the ?1, ?2, ?3, ?4 heavy chain of human IgGs, and the Fc of human IgG. There is no cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml or 50ng/ml of Anti-gamma Heavy Chain Antibody [RM116] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121291) - Antibodies.comEnlarge Image
Figure 3: Anti-gamma Heavy Chain Antibody [RM116] (A121312)
A titer ELISA using Anti-gamma Heavy Chain Antibody [RM116]. The plate was coated with different amounts of human IgG1. A serial dilution of Anti-gamma Heavy Chain Antibody [RM116] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-gamma Heavy Chain Antibody [RM116] (A121312) - Antibodies.comEnlarge Image
Figure 4: Anti-gamma Heavy Chain Antibody [RM116] (A121312)
Sandwich ELISA using Anti-gamma Heavy Chain Antibody [RM116] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody (1µg/ml), followed by an alkaline phosphatase conjugated streptavidin.
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