Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CCDS2, Epididymis secretory protein Li 20, GAMT_HUMAN, Guanidinoacetate N methyltransferase, Guanidinoacetate N-methyltransferase, HEL-S-20, PIG2, TP53I2
Figure 1: Western Blot - Anti-GAMT Antibody (A9930)
Western blot analysis of extracts of various cell lines, using Anti-GAMT Antibody (A9930) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-GAMT Antibody (A9930) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A549 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of A549 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of Hep G2 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of Hep G2 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-GAMT Antibody (A9930) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
