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Anti-GATAD2B Antibody (A16332)

Western Blot - Anti-GATAD2B Antibody (A16332) - Antibodies.com Immunohistochemistry - Anti-GATAD2B Antibody (A16332) - Antibodies.com Western Blot - Anti-GATAD2B Antibody (A16332) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to GATAD2B for WB, IHC and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-GATAD2B Antibody
Description
Rabbit polyclonal antibody to GATAD2B.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GATAD2B (NP_065750.1).
Sequence
MDRMTEDALRLNLLKRSLDPADERDDVLAKRLKMEGHEAMERLKMLALLKRKDLANLEVPHELPTKQDGSGVKGYEEKLNGNLRPHGDNRTAGRPGKENINDEPVDMSARRSEPERGRLT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FLJ37346, GATA zinc finger domain containing 2B, GATA zinc finger domain containing protein 2B, GATA zinc finger domain-containing protein 2B, KIAA1150, MGC138257, MGC138285, mKIAA1150, p66 beta, p66/p68, P66beta, P66B_HUMAN, RGD1308533, transcription repressor p66 beta component of the MeCP1 complex, Transcriptional repressor p66 beta, Transcriptional repressor p66-beta
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-GATAD2B Antibody (A16332) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-GATAD2B Antibody (A16332)
Western blot analysis of extracts of various cell lines, using Anti-GATAD2B Antibody (A16332) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-GATAD2B Antibody (A16332) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-GATAD2B Antibody (A16332)
Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-GATAD2B Antibody (A16332) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot - Anti-GATAD2B Antibody (A16332) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-GATAD2B Antibody (A16332)
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATAD2B Antibody (A16332). This Western blot was performed on the immunoprecipitate using Anti-GATAD2B Antibody (A16332) at a dilution of 1:1000.
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