Anti-GCH1 Antibody (A8676)

Name: Anti-GCH1 Antibody
Brand: Antibodies.com
SKU: A8676
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to GCH1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GCH1 Antibody

Description

Rabbit polyclonal antibody to GCH1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 55-250 of human GCH1 (NP_000152.1).

Sequence

GERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

dystonia 14, DYT 5, DYT14, DYT5, DYT5a, GCH, GCH 1, GCH1_HUMAN, GTP CH 1, GTP CH I, GTP cyclohydrolase 1, GTP cyclohydrolase 1 (dopa responsive dystonia), GTP cyclohydrolase I, GTP-CH-I, GTPCH 1, GTPCH1, Guanosine 5' triphosphate cyclohydrolase I, HPABH4B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GCH1 Antibody (A8676)

Western blot analysis of various lysates, using Anti-GCH1 Antibody (A8676) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 2: Immunofluorescence - Anti-GCH1 Antibody (A8676)

Immunofluorescence analysis of HeLa cells using Anti-GCH1 Antibody (A8676) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-GCH1 Antibody (A8676)

Immunofluorescence analysis of NIH/3T3 cells using Anti-GCH1 Antibody (A8676) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-GCH1 Antibody (A8676)

Immunofluorescence analysis of PC-12 cells using Anti-GCH1 Antibody (A8676) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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