Anti-GDNF Antibody (A305143)

Name: Anti-GDNF Antibody
Brand: Antibodies.com
SKU: A305143
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to GDNF for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GDNF Antibody

Description

Rabbit polyclonal antibody to GDNF.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human, Rat

Immunogen

Synthetic peptide from the mid-protein of Human GDNF.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Astrocyte derived trophic factor, Astrocyte derived trophic factor 1, Astrocyte-derived trophic factor, ATF, ATF 1, ATF 2, ATF1, ATF2, GDNF_HUMAN, Glial cell derived neurotrophic factor, Glial cell line derived neurotrophic factor , Glial cell line-derived neurotrophic factor, Glial derived neurotrophic factor, HFB1 GDNF, hGDNF, HSCR3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunohistochemistry - Anti-GDNF Antibody (A305143)

Immunohistochemistry analysis of human brain, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-GDNF Antibody (A305143) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.

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Figure 2: Western Blot - Anti-GDNF Antibody (A305143)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~23.7 kDa GDNF protein using Anti-GDNF Antibody (A305143) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer.

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Figure 3: Immunofluorescence - Anti-GDNF Antibody (A305143)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GDNF Antibody (A305143), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Vesicles, Nucleoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite.

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