Anti-GFPT1 Antibody [ARC2814] (A306761)

Name: Anti-GFPT1 Antibody [ARC2814]
Brand: Antibodies.com
SKU: A306761

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This product is discontinued

Anti-GFPT1 Antibody [ARC2814] (A306761) has been discontinued and is no longer available.

View all Anti-GFPT1 Antibodies.

Specifications

Name

Anti-GFPT1 Antibody [ARC2814]

Description

Rabbit monoclonal [ARC2814] antibody to GFPT1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-699 of human GFPT1 (Q06210).

Sequence

LVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2814

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CMS12, CMSTA1, D-fructose-6-phosphate amidotransferase 1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, GFPT1_HUMAN, Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1, Glutamine--fructose-6-phosphate transaminase 1, Glutamine:fructose 6 phosphate amidotransferase 1, Hexosephosphate aminotransferase 1, MSLG

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-GFPT1 Antibody [ARC2814] (A306761)

Western blot analysis of extracts of Mouse large intestine, using Anti-GFPT1 Antibody [ARC2814] (A306761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-GFPT1 Antibody [ARC2814] (A306761)

Western blot analysis of extracts of various cell lines, using Anti-GFPT1 Antibody [ARC2814] (A306761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Western Blot - Anti-GFPT1 Antibody [ARC2814] (A306761)

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Figure 4: Immunohistochemistry - Anti-GFPT1 Antibody [ARC2814] (A306761)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GFPT1 Antibody [ARC2814] (A306761) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.