Anti-Giantin Antibody (A306214)

Name: Anti-Giantin Antibody
Brand: Antibodies.com
SKU: A306214
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Giantin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Golgi Marker

Specifications

Name

Anti-Giantin Antibody

Description

Rabbit polyclonal antibody to Giantin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1704-1797 of human GOLGB1 (NP_004478.3).

Sequence

HPAGDTAKECMETLLSSNASMKEELERVKMEYETLSKKFQSLMSEKDSLSEEVQDLKHQIEGNVSKQANLEATEKHDNQTNVTEEGTQSIPGET

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

372 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

372 kDa Golgi complex associated protein, 372 kDa Golgi complex-associated protein, GCP, GCP372, GOGB1_HUMAN, GOLGB1, Golgi autoantigen golgin subfamily a member 1, Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1, Golgi autoantigen, golgin subfamily b, macrogolgin 1, Golgi complex associated protein 372kDa, Golgi complex-associated protein 372kDa, Golgin B1, Golgin B1, golgi integral membrane protein, Golgin subfamily B member 1, GOLIM1, Macrogolgin, MGC33154

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Giantin Antibody (A306214)

Western blot analysis of extracts of HeLa cells, using Anti-Giantin Antibody (A306214) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Giantin Antibody (A306214)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Giantin Antibody (A306214) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Giantin Antibody (A306214)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Giantin Antibody (A306214) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Giantin Antibody (A306214)

Immunofluorescence analysis of U2OS cells using Anti-Giantin Antibody (A306214) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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