Anti-GIT1 Antibody (A92785)

Name: Anti-GIT1 Antibody
Brand: Antibodies.com
SKU: A92785
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to GIT1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-GIT1 Antibody

Description

Rabbit polyclonal antibody to GIT1.

Applications

WB, ICC/IF

Dilutions

WB: 1:200-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).

Sequence

EIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARF GAP GIT1, ARF GTPase activating protein GIT1, ARF GTPase-activating protein GIT1, CAT 1, CAT-1, CaT1, Cool associated and tyrosine phosphorylated protein 1, Cool-associated and tyrosine-phosphorylated protein 1, G protein coupled receptor kinase interacting ArfGAP 1, G protein coupled receptor kinase interactor 1, G protein-coupled receptor kinase-interactor 1, GIT1_HUMAN, GRK interacting protein 1, GRK-interacting protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-GIT1 Antibody (A92785)

Western blot analysis of extracts of various cell lines, using Anti-GIT1 Antibody (A92785) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-GIT1 Antibody (A92785)

Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using Anti-GIT1 Antibody (A92785) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.