Anti-Gli1 Antibody [ARC59108] (A309375)

Name: Anti-Gli1 Antibody [ARC59108]
Brand: Antibodies.com
SKU: A309375

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This product is discontinued

Anti-Gli1 Antibody [ARC59108] (A309375) has been discontinued and is no longer available.

View all Anti-Gli1 Antibodies.

Specifications

Name

Anti-Gli1 Antibody [ARC59108]

Description

Rabbit monoclonal [ARC59108] antibody to Gli1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human GLI1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59108

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GLI, Gli 1, GLI family zinc finger 1, GLI Kruppel family member 1, GLI1_HUMAN, Glioma associated oncogene 1, Glioma associated oncogene homolog, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zfp5, Zinc finger protein GLI 1, Zinc finger protein GLI1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Gli1 Antibody [ARC59108] (A309375)

Western blot analysis of A-549, using Anti-Gli1 Antibody [ARC59108] (A309375) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunofluorescence analysis of Neuro-2a cells using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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