Anti-Glucagon Antibody [ARC56934] (A309735)

Western blot analysis of various lysates, using Anti-Glucagon Antibody [ARC56934] (A309735) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded human pancreas using Anti-Glucagon Antibody [ARC56934] (A309735) at a dilution of 1:50000/100000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Confocal imaging of human pancreas using Anti-Glucagon Antibody [ARC56934] (A309735), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

Confocal imaging of mouse pancreas using Anti-Glucagon Antibody [ARC56934] (A309735), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.