Anti-GLUD1 + GLUD2 Antibody [ARC53980] (A309225)

Western blot analysis of extracts of various cell lines, using Anti-GLUD1 + GLUD2 Antibody [ARC53980] (A309225) at 1:110000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GLUD1 + GLUD2 Antibody [ARC53980] (A309225) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Confocal imaging of Hep G2 cells using Anti-GLUD1 + GLUD2 Antibody [ARC53980] (A309225), at a dilution of 1:200, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.