GLUT1 Cell Based ELISA Kit (A103098)

GLUT1 Cell Based ELISA Kit

Cell-based (qualitative)

This GLUT1 Cell Based ELISA Kit allows for the detection of GLUT1 and the effects that certain stimulation conditions have on GLUT1 expression in different cell lines. Qualitative determination of GLUT1 concentration is achieved by an indirect ELISA format. In essence, the GLUT1 is captured by Anti-GLUT1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this GLUT1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity), CSE, DYT17, DYT18, DYT9, EIG12, erythrocyte/brain, Erythrocyte/hepatoma glucose transporter, facilitated glucose transporter member 1, Glucose transporter 1, Glucose Transporter GLUT1, Glucose transporter type 1, Glucose transporter type 1, erythrocyte/brain, GLUT, GLUT-1, GLUT1DS, GLUTB, GT1, GTG1, Gtg3, GTR1_HUMAN, HepG2 glucose transporter, HTLVR, Human T cell leukemia virus (I and II) receptor, MGC141895, MGC141896, PED, RATGTG1, Receptor for HTLV 1 and HTLV 2, SLC2A1, Solute carrier family 2, Solute carrier family 2 (facilitated glucose transporter), member 1, Solute carrier family 2, facilitated glucose transporter member 1