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Anti-Glutaminase Antibody (A11694)

Western Blot - Anti-Glutaminase Antibody (A11694) - Antibodies.com Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.com Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.com Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Glutaminase for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Glutaminase Antibody
Description
Rabbit polyclonal antibody to Glutaminase.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS)) (NP_055720.3).
Sequence
KVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
65 - 73 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AAD20, DKFZp686O15119, FLJ10358, GAC, GAM, GLS, GLS1, GLSK_HUMAN, Glutaminase C, Glutaminase kidney isoform, Glutaminase phosphate activated, K-glutaminase, KGA, KIAA0838, L-glutamine amidohydrolase, mitochondrial
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Glutaminase Antibody (A11694) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Glutaminase Antibody (A11694)
Western blot analysis of extracts of various cell lines, using Anti-Glutaminase Antibody (A11694) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Glutaminase Antibody (A11694)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Glutaminase Antibody (A11694)
Immunofluorescence analysis of PC-12 cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Glutaminase Antibody (A11694) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Glutaminase Antibody (A11694)
Immunofluorescence analysis of SH-SY5Y cells using Anti-Glutaminase Antibody (A11694) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).
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