Rabbit polyclonal antibody to Glutathione Synthetase.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human Glutathione Synthetase (Glutathione Synthetase (GSS)) (NP_000169.1).
Figure 1: Western Blot - Anti-Glutathione Synthetase Antibody (A92968)
Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using Anti-Glutathione Synthetase Antibody (A92968) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-Glutathione Synthetase Antibody (A92968) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-Glutathione Synthetase Antibody (A92968) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-Glutathione Synthetase Antibody (A92968) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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