Anti-GNAI3 Antibody (A92943)

Name: Anti-GNAI3 Antibody
Brand: Antibodies.com
SKU: A92943
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to GNAI3 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-GNAI3 Antibody

Description

Rabbit polyclonal antibody to GNAI3.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).

Sequence

MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

87U6, FLJ26559, G protein alpha inhibiting 3, G(i) alpha 3, G(i) alpha-3, GNAI3_HUMAN, Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3, Guanine nucleotide binding protein G(k) alpha subunit, Guanine nucleotide-binding protein G(k) subunit alpha, OTTHUMP00000013368

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GNAI3 Antibody (A92943)

Western blot analysis of extracts of various cell lines, using Anti-GNAI3 Antibody (A92943) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-GNAI3 Antibody (A92943)

Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using Anti-GNAI3 Antibody (A92943) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunofluorescence - Anti-GNAI3 Antibody (A92943)

Immunofluorescence analysis of L929 cells using Anti-GNAI3 Antibody (A92943) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-GNAI3 Antibody (A92943)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-GNAI3 Antibody (A92943). This Western blot was performed on the immunoprecipitate using Anti-GNAI3 Antibody (A92943) at a dilution of 1:1000.

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