Anti-GnRH Antibody (A9729)

Name: Anti-GnRH Antibody
Brand: Antibodies.com
SKU: A9729
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to GnRH for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GnRH Antibody

Description

Rabbit polyclonal antibody to GnRH.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human GNRH1 (NP_001076580.1).

Sequence

QHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQPRSPLRDLKGALESLIEEETGQKKI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GnRH associated peptide 1, GNRH1, Gonadotrophin releasing hormone 1, GRH, HH12, LHRH, LNRH, Luliberin I, Luteinizing releasing hormone, Lutenizing hormome releasing hormone, Progonadoliberin 1, Progonadoliberin I, Prolactin release inhibiting factor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GnRH Antibody (A9729)

Western blot analysis of extracts of Mouse lung, using Anti-GnRH Antibody (A9729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-GnRH Antibody (A9729)

Western blot analysis of extracts of Rat kidney, using Anti-GnRH Antibody (A9729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-GnRH Antibody (A9729)

Immunofluorescence analysis of SH-SY5Y cells using Anti-GnRH Antibody (A9729) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-GnRH Antibody (A9729)

Immunofluorescence analysis of NIH/3T3 cells using Anti-GnRH Antibody (A9729) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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