Anti-GRK2 Antibody [ARC1013] (A309171)

Name: Anti-GRK2 Antibody [ARC1013]
Brand: Antibodies.com
SKU: A309171
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1013] antibody to GRK2 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-GRK2 Antibody [ARC1013]

Description

Rabbit monoclonal [ARC1013] antibody to GRK2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 590-689 of human GRK2 (P25098).

Sequence

WRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1013

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADRBK1, Adrenergic beta receptor kinase 1, ARBK1_HUMAN, BARK, BARK1, Beta adrenergic receptor kinase 1, Beta ARK 1, Beta ARK1, Beta-adrenergic receptor kinase 1, Beta-ARK-1, FLJ16718, G protein coupled receptor kinase 2, G-protein coupled receptor kinase 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GRK2 Antibody [ARC1013] (A309171)

Western blot analysis of extracts of various cell lines, using Anti-GRK2 Antibody [ARC1013] (A309171) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-GRK2 Antibody [ARC1013] (A309171)

Western blot analysis of extracts from wild type (WT) and GRK2 knockout (KO) HeLa cells, using Anti-GRK2 Antibody [ARC1013] (A309171) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-GRK2 Antibody [ARC1013] (A309171)

Immunofluorescence analysis of HeLa cells using Anti-GRK2 Antibody [ARC1013] (A309171) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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