Anti-GRP94 Antibody [9G10] (A305089)

Name: Anti-GRP94 Antibody [9G10]
Brand: Antibodies.com
SKU: A305089
Price: 450.0 USD
Availability: InStock

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$450

Rat monoclonal [9G10] antibody to GRP94 for WB, ICC/IF, IP and Flow Cytometry.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GRP94 Antibody [9G10]

Description

Rat monoclonal [9G10] antibody to GRP94.

Specificity

This antibody does not detect human HSP90, Grp74, or GrpE from E. coli.

Applications

WB, ICC/IF, IP, Flow Cytometry

Dilutions

WB: 1:2,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Horse, Monkey, Porcine, Rabbit, Sheep, Xenopus

Immunogen

Native Grp94, isolated and purified from chicken oviducts.

Host

Rat

Clonality

Monoclonal

Clone ID

9G10

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~98 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL_HUMAN, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96)

Isotype Controls

Suitable Secondaries

See all Anti-Rat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GRP94 Antibody [9G10] (A305089)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of GRP94 protein using Anti-GRP94 Antibody [9G10] (A305089) at 1:1,000. The secondary antibody used was HRP Goat Anti-Rat.

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Figure 2: Immunofluorescence - Anti-GRP94 Antibody [9G10] (A305089)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP94 Antibody [9G10] (A305089), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rat (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 3: Immunofluorescence - Anti-GRP94 Antibody [9G10] (A305089)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP94 Antibody [9G10] (A305089), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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