Anti-GTPase HRAS Antibody [H-Ras-03] (A280653)

Anti-GTPase HRAS Antibody [H-Ras-03]

Mouse monoclonal [H-Ras-03] antibody to GTPase HRAS.

This antibody recognises the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes. H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi. Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation. Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting.

ELISA, WB

WB: 0.5 - 1 µg/ml

Human, Mouse, Rat

Synthetic peptide sequence DIHQYREQIKRVKDSDDC of human H-Ras.

DIHQYREQIKRVKDSDDC

Mouse

Monoclonal

H-Ras-03

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburn et al. 1990).H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006).Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluoto et al. 2011).

C BAS/HAS, c H ras, C HA RAS1, c has/bas p21 protein, c ras Ki 2 activated oncogene, c-H-ras, CTLO, GTP and GDP binding peptide B, GTPase HRas, N-terminally processed, H Ras 1, H RASIDX, H-Ras-1, Ha Ras, Ha Ras1 proto oncoprotein, Ha-Ras, HAMSV, Harvey rat sarcoma viral oncogene homolog, Harvey rat sarcoma viral oncoprotein, HRAS, HRAS1, K ras, N ras, p19 H RasIDX protein, p21ras, Ras family small GTP binding protein H Ras, RASH1, RASH_HUMAN, Transformation gene oncogene HAMSV, Transforming protein p21, v Ha ras Harvey rat sarcoma viral oncogene homolog, VH Ras, vHa RAS