Anti-GUCY1B3 Antibody [ARC2071] (A308007)

Name: Anti-GUCY1B3 Antibody [ARC2071]
Brand: Antibodies.com
SKU: A308007
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2071] antibody to GUCY1B3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GUCY1B3 Antibody [ARC2071]

Description

Rabbit monoclonal [ARC2071] antibody to GUCY1B3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Guanylyl Cyclase beta 1 (GUCY1B3) (Q02153).

Sequence

MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2071

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

71 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GC-S-beta-1, GC-SB3, GCbeta1, GCS beta 1, GCS beta 3, GCS-beta-1, GCS-beta-3, GCYB1_HUMAN, guanylate cyclase 1, soluble, beta 3, Guanylate cyclase soluble beta 1 chain, Guanylate cyclase soluble subunit beta-1, Guanylate cyclase soluble subunit beta-3, Guanylyl Cyclase beta 1, GUC1B3, GUCSB3, GUCY1B1, Gucy1b1;, SGC, Soluble guanylate cyclase small subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GUCY1B3 Antibody [ARC2071] (A308007)

Western blot analysis of extracts of various cell lines, using Anti-GUCY1B3 Antibody [ARC2071] (A308007) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-GUCY1B3 Antibody [ARC2071] (A308007)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-GUCY1B3 Antibody [ARC2071] (A308007) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-GUCY1B3 Antibody [ARC2071] (A308007)

Immunofluorescence analysis of NIH-3T3 cells using Anti-GUCY1B3 Antibody [ARC2071] (A308007) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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