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Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)

Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.com
This product is discontinued

Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) has been discontinued and is no longer available.

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Name
Anti-HA Tag Antibody [AMC0512] (HRP)
Description
Mouse monoclonal [AMC0512] antibody to HA Tag.
Applications
WB
Dilutions
WB: 1:500-1:5,000
Reactivity
Species Independent
Immunogen
A synthetic peptide corresponding to HA tag.
Sequence
YPYDVPDYA
Host
Mouse
Clonality
Monoclonal
Clone ID
AMC0512
Isotype
IgG1
Light Chains
kappa
Conjugate

HRP

Purification
Affinity purification.
Molecular Weight
53 kDa (CD95) / 65 kDa (CD2) / 53 kDa (SCRN1) 42 kDa (WDR77) / 45 kDa (NR3C2)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)
Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein, using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)
Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein, 293F transfected with CD95-N Protein using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-HA Tag Antibody [AMC0512] (HRP) (A93073)
Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using Anti-HA Tag Antibody [AMC0512] (HRP) (A93073) at 1:5,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection was with an ECL Basic Kit. Exposure time: 90s.
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