Anti-Hamartin Antibody (A12695)

Name: Anti-Hamartin Antibody
Brand: Antibodies.com
SKU: A12695
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Hamartin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Hamartin Antibody

Description

Rabbit polyclonal antibody to Hamartin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human TSC1 (NP_000359.1).

Sequence

QAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

kiaa0243, LAM, TSC, Tsc1, Tsc1 gene, TSC1_HUMAN, Tuberous sclerosis 1, Tuberous sclerosis 1 protein, tumor suppressor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Hamartin Antibody (A12695)

Western blot analysis of extracts of Rat spleen, using Anti-Hamartin Antibody (A12695) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Hamartin Antibody (A12695)

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Anti-Hamartin Antibody (A12695) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Hamartin Antibody (A12695)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-Hamartin Antibody (A12695) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Hamartin Antibody (A12695)

Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Anti-Hamartin Antibody (A12695) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.