Anti-HCN2 Antibody [S71] (A304767)

Name: Anti-HCN2 Antibody [S71]
Brand: Antibodies.com
SKU: A304767
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S71] antibody to HCN2 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HCN2 Antibody [S71]

Description

Mouse monoclonal [S71] antibody to HCN2.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with HCN1.

Immunogen

Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2.

Host

Mouse

Clonality

Monoclonal

Clone ID

S71

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~95 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCNG-2, BCNG2, Brain cyclic nucleotide gated channel 2, Brain cyclic nucleotide-gated channel 2, HAC1, HCN2_HUMAN, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-HCN2 Antibody [S71] (A304767)

Western blot analysis of mouse brain showing detection of ~95 kDa HCN2 protein using Anti-HCN2 Antibody [S71] (A304767) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Ladder. Lane 2: mouse brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~95 kDa.

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Figure 2: Immunohistochemistry - Anti-HCN2 Antibody [S71] (A304767)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HCN2 Antibody [S71] (A304767) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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Figure 3: Western Blot - Anti-HCN2 Antibody [S71] (A304767)

Western blot analysis of rat brain membrane lysate showing detection of HCN2 protein using Anti-HCN2 Antibody [S71] (A304767) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 4: Immunofluorescence - Anti-HCN2 Antibody [S71] (A304767)

Immunocytochemistry/Immunofluorescence analysis of human differentiated SH-SY5Y, using Anti-HCN2 Antibody [S71] (A304767), at 1:100. The secondary antibody used was AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue).(A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.