Anti-HDAC1 Antibody (A12564)

Name: Anti-HDAC1 Antibody
Brand: Antibodies.com
SKU: A12564
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to HDAC1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody Nuclear Loading Control

Specifications

Name

Anti-HDAC1 Antibody

Description

Rabbit polyclonal antibody to HDAC1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).

Sequence

SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686H12203, GON 10, HD1, HDAC 1, HDAC1_HUMAN, Histone deacetylase 1, Reduced potassium dependency yeast homolog like 1, RPD3, RPD3L1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-HDAC1 Antibody (A12564)

Western blot analysis of extracts of various cell lines, using Anti-HDAC1 Antibody (A12564) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-HDAC1 Antibody (A12564)

Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using Anti-HDAC1 Antibody (A12564) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-HDAC1 Antibody (A12564)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-HDAC1 Antibody (A12564) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HDAC1 Antibody (A12564)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-HDAC1 Antibody (A12564) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.