Anti-HDAC1 Antibody [ARC0050] (A307930)

Name: Anti-HDAC1 Antibody [ARC0050]
Brand: Antibodies.com
SKU: A307930

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This product is discontinued

Anti-HDAC1 Antibody [ARC0050] (A307930) has been discontinued and is no longer available.

View all Anti-HDAC1 Antibodies.

Specifications

Name

Anti-HDAC1 Antibody [ARC0050]

Description

Rabbit monoclonal [ARC0050] antibody to HDAC1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HDAC1 (Q13547).

Sequence

MTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0050

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686H12203, GON 10, HD1, HDAC 1, HDAC1_HUMAN, Histone deacetylase 1, Reduced potassium dependency yeast homolog like 1, RPD3, RPD3L1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-HDAC1 Antibody [ARC0050] (A307930)

Western blot analysis of extracts of various cell lines, using Anti-HDAC1 Antibody [ARC0050] (A307930) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-HDAC1 Antibody [ARC0050] (A307930)

Western blot analysis of extracts from wild type (WT) and HDAC1 knockout (KO) 293T cells, using Anti-HDAC1 Antibody [ARC0050] (A307930) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-HDAC1 Antibody [ARC0050] (A307930)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HDAC1 Antibody [ARC0050] (A307930) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HDAC1 Antibody [ARC0050] (A307930)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-HDAC1 Antibody [ARC0050] (A307930) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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