Anti-HDAC8 Antibody [ARC1331] (A307212)

Name: Anti-HDAC8 Antibody [ARC1331]
Brand: Antibodies.com
SKU: A307212
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1331] antibody to HDAC8 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-HDAC8 Antibody [ARC1331]

Description

Rabbit monoclonal [ARC1331] antibody to HDAC8.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC8 (Q9BY41).

Sequence

MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1331

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDA07, CDLS5, HD 8, HD8, HDAC 8, HDAC8_HUMAN, HDACL 1, HDACL1, Histone deacetylase 8, Histone deacetylase like 1, MRXS6, RPD 3, RPD3, WTS

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-HDAC8 Antibody [ARC1331] (A307212)

Western blot analysis of extracts of various cell lines, using Anti-HDAC8 Antibody [ARC1331] (A307212) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-HDAC8 Antibody [ARC1331] (A307212)

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Figure 3: Western Blot - Anti-HDAC8 Antibody [ARC1331] (A307212)

Western blot analysis of extracts from wild type (WT) and HDAC8 knockout (KO) 293T cells, using Anti-HDAC8 Antibody [ARC1331] (A307212) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 4: Immunohistochemistry - Anti-HDAC8 Antibody [ARC1331] (A307212)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-HDAC8 Antibody [ARC1331] (A307212) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.