Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HBP, HDL binding protein, HDL-binding protein, high density lipoprotein binding protein, high density lipoprotein binding protein (vigilin), High density lipoprotein-binding protein, PRO2900, VGL, Vigilin, VIGLN_HUMAN
Figure 1: Western Blot - Anti-HDLBP Antibody (A88332)
Western blot analysis of extracts of various cell lines, using Anti-HDLBP Antibody (A88332) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-HDLBP Antibody (A88332) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-HDLBP Antibody (A88332) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-HDLBP Antibody (A88332) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Anti-HDLBP Antibody (A88332) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
