Armenian hamster monoclonal [22F6] antibody to ZNFN1A2/HELIOS (PE).
Specificity
This antibody recognizes an intracellular epitope, between amino acids 51-107, of Helios, a transcription factor expressed in some hematopoietic stem cells and at high levels in thymic-derived regulatory T cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Mouse, Human
Immunogen
Synthetic peptide corresponding to amino acids 51-107 of Helios.
Host
Armenian Hamster
Clonality
Monoclonal
Clone ID
22F6
Isotype
IgG
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Human peripheral blood lymphocytes were stained with Anti-CD4 Antibody (Pacific Blue), fixed and permeabilized with Treg Detection Kit (Miltenyi Cat #: 130-094-163), and then intracellulary stained with Anti-Helios Antibody (A86297).
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